Ginkgolide B inhibition of the inflammatory protein expression associated with reduce NOX4 expression and NF-kB activation in ox-LDL-stimulated HUVECs
Ruomei Qi
Beijing Institute of Geriatrics, Beijing Hospital and Key laboratory of Geriatrics Ministry of Health, Beijing, 100730, China
Abstract:
Object: The aim of the current study is to investigate the effect of PAF antagonist, ginkgolide B and CV3988 on the expression of NADPH, inflammatory expression in ox-LDL-stimulated human vascular endothelial cells (HUVECs).
Methods: HUVECs were pretreated with ginkgolide B or CV3988 and then exposed to ox-LDL. RT-PCR was employed to examine gene expression. Western blot and ELISA was used to examine protein expression.
Results: Pretreatment of Ginkgolide B blocked ox-LDL induced expression of NOX4 and ROS production, but not CV3988. Moreover, both ginkgolide B and CV3988 inhibited ox-LDL induced MCP-1 mRNA. The protein level of MCP-1 and ICAM-1 expression was also reduced in HUVECs. Furthermore, the nuclear extracts were prepared and NF-kB p65 was examined by Western blotting, it was found that both ginkgolide B and CV3988 inhibited ox-LDL induced NF-kB p65 translocation into the nucleus.
Conclusion: Although both ginkgolide B and CV3988 are platelet-activating factor inhibitors and they can inhibit the expression of inflammatory proteins through blocking NF-kB translocation, ginkgolide B may have further activity in oxidative stress.
